BREISGAU, Germany, Nov. 10, 2021 - Potchula kuchuluka kwa kukana kwa maantibayotiki komwe kukuchulukirachulukira padziko lonse lapansi, ofufuza ochokera ku Fraunhofer Institute for Physical Measurement Techniques (Fraunhofer IPM), akugwira ntchito limodzi ndi a Ludwig Maximilian University of Munich, apanga njira yofulumira kwambiri. kupeza tizilombo toyambitsa matenda tosamva mankhwala ambiri. Njirayi ndi yovuta kwambiri kuti igwiritse ntchito molekyu imodzi ya DNA kuti izindikire tizilombo toyambitsa matenda.

Kupeza maantibayotiki ogwira mtima kwambiri nthawi zambiri kumafuna kudziwa zambiri zamtundu wa bakiteriya, zomwe sizipezeka kwachipatala. Kuyesa kwa labu kumafunika, zomwe zimawonjezera nthawi komanso zovuta pakufufuza. Njira yopangidwa ndi ochita kafukufuku imafulumizitsa ndondomekoyi, pogwiritsa ntchito chipangizo cha microfluidic kuti chizindikire ndi kusanthula mamolekyu amodzi. Cholinga cha pulojekiti ya SiBoF (zizindikiro za ma sign of fluorescence assays in molecular diagnostics) zagona pa njira yosavuta yodziwira malo osamalira. Ofufuzawo akuyembekeza kuti nsanjayo igwiritsidwe ntchito ngati gawo lazachipatala m'mawodi am'chipatala kapena m'zachipatala ngati njira ina yowunikiranso kusanthula kwapolymerase chain reaction.
Chipangizo chophatikizika chodziwira tizilombo tosamva mankhwala ambiri chimachita magawo onse azomwe zimachitika ndipo chimapereka zotsatira mkati mwa ola limodzi. Ngakhale molekyu imodzi ya DNA ndiyokwanira kuzindikira. Mwachilolezo cha Fraunhofer IPM
Gulu la ofufuza ku Germany lakonza njira yodziwira msanga tizilombo tosamva mankhwala. Njirayi imagwiritsa ntchito chipangizo chophatikizika chomwe chimachita magawo onse azomwe zimachitika zokha ndipo chimapereka zotsatira mkati mwa ola limodzi. Ngakhale molekyu imodzi ya DNA ndiyokwanira kuzindikira. Mwachilolezo cha Fraunhofer IPM.
Pulatifomu yoyeserera yonyamula, yaying'ono imakhala ndi makina opangira madzi, momwe ma reagents onse ofunikira amasungidwa. Chip chopangidwa ndi jekeseni cha microfluidic chimaphatikizidwa mu kabati mu dongosolo loyesera, komwe amaperekedwa ndi ma reagents ofunikira kudzera mu dongosolo la fluidic musanayambe kufufuza kwa kuwala.

“Timazindikira mbali ina ya DNA ya tizilombo toyambitsa matenda. Pogwiritsa ntchito njira yathu yatsopano, ngakhale molekyu imodzi ya DNA yomwe imamangiriza kumalo enaake pa microfluidic chip ndiyokwanira kuchita izi. Njira zamadzimadzi zimaphatikizidwa mu chip - malo omwe amakhala ndi malo omangira tizilombo toyambitsa matenda," adatero Benedikt Hauer, woyang'anira polojekiti komanso wasayansi wofufuza ku Fraunhofer IPM.

Chipangizocho chili ndi maikulosikopu ya miniaturized high-resolution fluorescence. Mapulogalamu osanthula zithunzi opangidwa mwapadera amazindikiritsa mamolekyu amodzi, zomwe zimapangitsa kuti mamolekyu omwe atengedwa awerengedwe kuti apereke zotsatira zochulukirapo. Fluorescence imalimbikitsidwa pogwiritsa ntchito ma LED, omwe amamangiriridwa pansi pa cartridge yomwe ili ndi njira zamadzimadzi.

Nthawi zambiri, mamolekyu a DNA omwe amawunikira amazindikiridwa pogwiritsa ntchito zolembera za fulorosenti. Njira yatsopanoyi imagwiritsa ntchito tinyanga tokhala ndi mikanda ya kukula kwa nanometer, yomwe imakulitsa zizindikiro za kuwala kwa zolemberazi ndikuchotsa kudalira kukulitsa mankhwala kudzera pa PCR.

"Tinyanga zowala zimakhala ndi tinthu tating'onoting'ono tomwe timayang'ana kuwala m'dera laling'ono komanso timathandizira kutulutsa kuwala - monga momwe tinyanga tambiri tambiri timachitira ndi mafunde a wailesi," adatero Hauer. Zitsulo zachitsulo zimamangirizidwa ndi mankhwala pamwamba pa chip.

Kapangidwe ka mamolekyu a DNA, omwe ofufuzawo amati DNA origami, amasunga ma nanoparticles onse agolide m'malo mwake. Pakati pa nanoparticles, kapangidwe kameneka kamapereka malo omangirira pa molekyulu chandamale ndi cholembera cha fluorescence. Mapangidwe a patent amapereka maziko aukadaulo waukadaulo woyeserera.