BREISGAU, Germany, Mbudzi 10, 2021 - Vachitaura nezvekuwedzera kushorwa kwemishonga inorwisa mabhakitiriya kuri kuwedzera pasi rose, vaongorori veFraunhofer Institute for Physical Measurement Techniques (Fraunhofer IPM), vachishanda pamwe chete nevaya vekuLudwig Maximilian University yeMunich, vakagadzira nzira yekukurumidza. kuona utachiona husingaurayiwi nemishonga yakawanda. Nzira yacho inonzwisisika zvekukwanisa kushandisa molecule imwe chete yeDNA pakuona pathogen.

Kutsvaga mushonga unoshanda zvakanyanya kazhinji kunoda ruzivo nezve genome yebhakitiriya, iyo isingawanzo kuwanikwa pakurapa. Kuongororwa kweLab kunowanzo kudiwa, izvo zvinowedzera nguva uye kuoma pakutsvaga. Iyo nzira yakagadziridzwa nevanotsvaga inomhanyisa maitiro, vachishandisa microfluidic chip kuona uye kuongorora mamorekuru mamwe. Tarisiro yeSiBoF (signal boosters for fluorescence assays in molecular diagnostics) chirongwa chiri panzira iri nyore kushandisa yekutarisisa-yekutarisira. Vatsvaguri vanofungidzira kuti chikuva chiri kushandiswa sechikamu chekuona-che-kutarisirwa kuongororwa mumawadhi echipatara kana mune zvekurapa maitiro seimwe nzira kune yakamiswa polymerase chain reaction ongororo.
Iyo compact mudziyo wekuona hutachiona husingachinjiki nemishonga inoita otomatiki nhanho dzekuita uye inopa mhedzisiro mukati meawa imwe. Kunyange molecule imwe yeDNA yakakwana kuti ionekwe. Nekuda kweFraunhofer IPM
Chikwata
Iyo inotakurika, compact test chikuva yakashongedzerwa ine otomatiki fluidic system, mune inochengetwa reagents ese anodiwa. Iyo jekiseni-yakaumbwa microfluidic chip inosanganisirwa mudhirowa muyedzo system, kwainopihwa neinodiwa reagents kuburikidza neiyo fluidic system isati yaitwa optical analysis.

"Tinoona chikamu cheDNA strand yepathogen. Tichishandisa maitiro edu matsva, kunyangwe morekuru rimwe reDNA rinosungirira kune chaiyo saiti pane microfluidic chip rinokwana kuita izvi. Fluidic chiteshi chakabatanidzwa muchip - nzvimbo dzayo dzakashongedzwa nenzvimbo dzinosunga dzehutachiona chaihwo," akatsanangura kudaro Benedikt Hauer, maneja wepurojekiti uye musayendisiti wekutsvagisa kuFraunhofer IPM.

Iyo poindi-ye-kutarisira mudziyo ine miniaturized yakakwirira-resolution fluorescence maikorosikopu. Yakanyatso kugadziridzwa mufananidzo wekuongorora software inozivisa mamorekuru mamwe, ayo anogonesa akabatwa anotangwa mamorekuru kuverengerwa kuti ape mhedzisiro yehuwandu. Iyo fluorescence inokurudzirwa uchishandisa maLED, ayo anonamirwa pasi pekaturiji iyo ine fluidic chiteshi.

Kazhinji, mamorekuru eDNA anotangwa anoonekwa kuburikidza neakananga magirosi efluorescence. Iyo nzira nyowani inoshandisa antennas ane nanometer-saizi mabhero, ayo anokwidziridza masaini masaini eaya mamaki uye anobvisa kuvimba nemakemikari amplification kuburikidza nePCR.

"Antennas anopenya ane nanometer-saizi simbi zvidimbu zvinonangidzira mwenje munzvimbo diki uye zvakare zvinobatsira kuburitsa mwenje - sezvinoita macroscopic antennas nemasaisai eredhiyo," Hauer akadaro. Zvimedu zvesimbi zvinosungirirwa nekemikari kumusoro kwechip.

Chimiro chemamorekuru eDNA, icho vatsvakurudzi vakarondedzera seDNA origami, inobata maviri egoridhe nanoparticles panzvimbo. Pakati pe nanoparticles, chimiro chinopa inosunga saiti kune yakatarisana chinangwa molecule uye fluorescence marker. Iyo patent dhizaini inopa hwaro hweinoveli assay tekinoroji.