HEIDELBERG, Germany, Okutobala 4, 2021 - Njira yomwe gulu la Prevedel linapanga ku European Molecular Biology Laboratory (EMBL) imalola asayansi kuti azitha kuwona ma neuron amoyo mkati mwa ubongo - kapena selo lina lililonse lobisika mkati mwa mnofu. Njirayi idakhazikitsidwa ndi ma microscopy atatu-photon ndi ma optic adaptive.

Njirayi imawonjezera kuthekera kwa asayansi kuwona ma astrocyte akupanga calcium yoyendetsedwa m'miyala yayikulu ya kotekisi, ndikuwonanso maselo ena aliwonse a neural mu hippocampus, dera laubongo lomwe limathandizira kukumbukira malo ndi kuyenda. Chodabwitsachi chimachitika pafupipafupi mu ubongo wa nyama zonse zamoyo. Lina Streich wochokera ku gulu la Prevedel ndi omwe adagwira nawo ntchito adatha kugwiritsa ntchito njirayi kuti adziwe tsatanetsatane wa ma cell osunthikawa pamalingaliro apamwamba kwambiri.
Galasi lopunduka lomwe limagwiritsidwa ntchito mu microscopy kuti liwonetse kuwala mkati mwazamoyo. Mwachilolezo cha Isabel Romero Calvo, EMBL.
Galasi lopunduka lomwe limagwiritsidwa ntchito mu microscopy kuti liwonetse kuwala mkati mwazamoyo. Gulu la EMBL limaphatikiza ma optic adaptive ndi ma microscopy atatu-photon kuti athandizire kuthekera kwa azachipatala kujambula mkati mwa hippocampus. Mwachilolezo cha Isabel Romero Calvo, EMBL.

Mu ma neuroscience, matupi aubongo nthawi zambiri amawoneka tating'onoting'ono tating'onoting'ono kapena zitsanzo za ex vivo zomwe zimafunikira kuti zidulidwe kuti ziwoneke - zonse zomwe zikuyimira zikhalidwe za thupi. Ntchito yabwinobwino yama cell amangochitika mu nyama zokha. Ubongo wa mbewa, komabe, ndi minofu yofalikira kwambiri, atero a Robert Prevedel. "Muubongo uwu, kuwala sikungayang'ane mosavuta, chifukwa kumalumikizana ndi zinthu zama cell," adatero. "Izi zimachepetsa momwe mungapangire chithunzi chokomera, ndipo zimapangitsa kuti zikhale zovuta kuyang'ana zazing'ono zomwe zili mkatikati mwaubongo pogwiritsa ntchito njira zachikhalidwe.

“Pogwiritsa ntchito maluso owonera tinthu tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tating'onoting'ono tomwe timagwira, ma photon awiri amalowetsedwa ndi molekyulu ya fluorescence nthawi iliyonse, ndipo mutha kutsimikiza kuti chisangalalo choyambitsidwa ndi cheza chimangokhala pang'ono. Koma kutalika kwa ma photon, kumakhala kotayika kwambiri chifukwa chobalalika. ”

Njira imodzi yogonjetsera izi ndikuwonjezera kutalika kwa ma photon osangalatsa kulowera ku infrared, komwe kumathandizira kuti mphamvu yama radiation yokwanira itengeke ndi fluorophore. Kuphatikiza apo, kugwiritsa ntchito ma photon atatu m'malo mwa awiri kumathandizira zithunzi zoperewera kupezeka mkatikati mwa ubongo. Vuto linanso lidatsalira: kuwonetsetsa kuti ma photon akuyang'ana, kuti chithunzi chonse chisasokonezeke.