BREISGAU, Kelemānia, Nov. 10, 2021 - Ke ʻōlelo nei i ka piʻi ʻana o ke kū'ē ʻana i nā lāʻau antibiotic i ka piʻi ʻana o ka honua, ua hoʻomohala nā mea noiʻi mai Fraunhofer Institute for Physical Measurement Techniques (Fraunhofer IPM), e hana pū ana me ka poʻe mai ka Ludwig Maximilian University of Munich, i hoʻomohala i kahi kaʻina no ka wikiwiki. ka ʻike ʻana i nā maʻi pathogens multidrug-resistant. He maʻalahi ke ala e hiki ai ke hoʻohana i hoʻokahi mole o DNA no ka ʻike pathogen.

ʻO ka loaʻa ʻana o ka lāʻau antibiotic maikaʻi loa e koi pinepine i ka ʻike e pili ana i ka genome o ka bacteria, ʻaʻole i loaʻa maʻamau i nā hana lapaʻau. Pono maʻamau ka hoʻāʻo ʻana o Lab, kahi e hoʻohui ai i ka manawa a me ka paʻakikī i ka ʻimi. ʻO ke ala i hoʻomohala ʻia e ka poʻe noiʻi e hoʻolalelale i ke kaʻina hana, me ka hoʻohana ʻana i kahi microfluidic chip e ʻike a nānā i nā molekala hoʻokahi. Aia ka manaʻo o ka SiBoF (nā hōʻailona hōʻailona no ka fluorescence assays in molecular diagnostics) papahana ma kahi ala maʻalahi e hoʻohana i ka ʻike maka-o-mālama. Manaʻo ka poʻe noiʻi e hoʻohana ʻia ke kahua ma ke ʻano o nā diagnostics point-of-care ma nā keʻena o ka haukapila a i ʻole nā ​​hana lapaʻau ma ke ʻano he koho ʻē aʻe i ka hoʻopaʻa ʻana i ka polymerase chain reaction analysis.
ʻO ka mīkini paʻa no ka ʻike ʻana i nā pathogens-resistant multidrug e hana maʻalahi i nā pae āpau o ka hopena a hāʻawi i ka hopena i loko o hoʻokahi hola. ʻO kahi mole DNA hoʻokahi i lawa no ka ʻike. Na Fraunhofer IPM
Ua
Hoʻolako ʻia ka paepae hoʻāʻo paʻa paʻa me kahi ʻōnaehana fluidic automated, kahi e mālama ʻia ai nā reagents pono a pau. Hoʻokomo ʻia ka chip microfluidic injection-molded i loko o kahi drawer i ka ʻōnaehana hoʻāʻo, kahi i hoʻolako ʻia me nā reagents e pono ai ma o ka ʻōnaehana fluidic ma mua o ka hoʻokō ʻana i ka nānā ʻana.

"ʻIke mākou i kahi ʻāpana o ke kaula DNA o ka pathogen. Ke hoʻohana nei i kā mākou kaʻina hana hou, ʻo kahi molela hoʻokahi o DNA e hoʻopaʻa ʻia i kahi kahua kikoʻī ma ka microfluidic chip ua lawa ia e hana i kēia. Hoʻohui ʻia nā kahawai Fluidic i loko o ka puʻupuʻu - ua hoʻokumu ʻia nā ʻili me nā wahi paʻa no nā pathogens kikoʻī, "i wehewehe ʻo Benedikt Hauer, ka luna papahana a me kahi ʻepekema noiʻi ma Fraunhofer IPM.

Hōʻike ʻia ka hāmeʻa point-of-care i kahi microscope fluorescence hoʻonā kiʻekiʻe. Hoʻomaopopo ʻia ka polokalamu hoʻonaʻauao kiʻi i kūkulu pono ʻia i nā molekala hoʻokahi, e hiki ai ke helu ʻia nā molekala i hopu ʻia e hāʻawi i kahi hopena quantitative. Hoʻoulu ʻia ka fluorescence me ka hoʻohana ʻana i nā LED, i hoʻopili ʻia ma lalo o ka pahu pahu i loaʻa nā kahawai wai.

ʻO ka maʻamau, ʻike ʻia nā molekole DNA i ʻike ʻia e nā māka fluorescence kikoʻī. Ke hoʻohana nei ke ʻano hou i nā antenna me nā peʻa nanometer-size, e hoʻonui ai i nā hōʻailona optical o kēia mau māka a hoʻopau i ka hilinaʻi ʻana i ka hoʻonui kemika ma o PCR.

"ʻO nā antenna optical he mau ʻāpana metala ka nui nanometer e hoʻokaʻawale i ka mālamalama i kahi wahi liʻiliʻi a kōkua pū i ka hoʻokuʻu ʻana i ka mālamalama - e like me nā antenna macroscopic me nā hawewe lekiō," wahi a Hauer. Hoʻopaʻa kemika nā ʻāpana metala i ka ʻili o ka chip.

ʻO kahi ʻano o nā molekala DNA, kahi i hoʻopaʻa ʻia e nā mea noiʻi he DNA origami, e paʻa i nā nanoparticles gula ʻelua. Ma waena o nā nanoparticles, hāʻawi ka hale i kahi kahua paʻa no kēlā me kēia mole paʻa a me kahi māka fluorescence. Hāʻawi ka hoʻolālā patent i ke kumu no ka ʻenehana assay novel.