BREISGAU, Jamus, Nuwamba 10, 2021 - Da yake ambaton karuwar juriya ga maganin rigakafi yana karuwa a duniya, masu bincike daga Fraunhofer Institute for Physical Measurement Techniques (Fraunhofer IPM), da ke aiki tare da waɗanda daga Jami'ar Ludwig Maximilian na Munich, sun ɓullo da tsari don sauri. gano ƙwayoyin cuta masu jurewa da yawa. Hanyar tana da hankali sosai don samun damar amfani da kwayar halittar DNA guda ɗaya don gano ƙwayoyin cuta.

Nemo maganin rigakafi mafi inganci sau da yawa yana buƙatar bayani game da kwayoyin halittar ƙwayoyin cuta, wanda yawanci ba ya samuwa a ayyukan likita. Gwajin Lab yawanci ana buƙata, wanda ke ƙara lokaci da rikitarwa ga binciken. Hanyar da masu binciken suka ɓullo da ita tana haɓaka aikin, ta yin amfani da guntu microfluidic don ganowa da kuma nazarin kwayoyin halitta guda ɗaya. Mayar da hankali na SiBoF (masu haɓaka sigina don ƙididdigar haske a cikin binciken ƙwayoyin cuta) aikin ya ta'allaka ne akan hanyar gano ma'anar kulawa mai sauƙin amfani. Masu binciken suna tsammanin za a yi amfani da dandamali a matsayin wani ɓangare na bincike-binciken kulawa a cikin sassan asibiti ko kuma a cikin ayyukan likita a matsayin madadin kafaffen binciken sarkar polymerase.
Ƙaƙƙarfan na'urar don gano ƙwayoyin cuta masu jure wa magunguna da yawa suna yin duk matakan amsawa ta atomatik kuma suna ba da sakamako cikin sa'a ɗaya. Ko da kwayar halittar DNA guda ɗaya ta isa a gano. Ladabi na Fraunhofer IPM
Tawagar masu bincike a Jamus sun ɓullo da wani tsari don gano ƙwayoyin cuta da yawa cikin sauri. Tsarin yana amfani da ƙaƙƙarfan na'ura wanda ke aiwatar da duk matakan amsawa ta atomatik kuma yana ba da sakamako cikin sa'a ɗaya. Ko da kwayar halittar DNA guda ɗaya ta isa a gano. Hoton Fraunhofer IPM.
Maɗaukaki, ɗan ƙaramin dandamalin gwaji sanye take da tsarin ruwa mai sarrafa kansa, wanda a cikinsa ake adana duk abubuwan da suka dace. Guntun microfluidic ɗin da aka ƙulla allura an haɗa shi a cikin aljihun tebur a cikin tsarin gwaji, inda aka ba da shi tare da abubuwan da suka dace ta hanyar tsarin ruwa kafin a gudanar da bincike na gani.

"Mun gano wani yanki na kwayar cutar ta DNA. Yin amfani da sabon tsarin mu, ko da kwayar halittar DNA guda ɗaya wanda ke ɗaure zuwa takamaiman rukunin yanar gizo akan guntun microfluidic ya isa yin wannan. An haɗa tashoshi masu ruwa da tsaki a cikin guntu - abubuwan da ke tattare da su tare da wuraren ɗaure don takamaiman ƙwayoyin cuta, "in ji Benedikt Hauer, manajan aikin kuma masanin kimiyyar bincike a Fraunhofer IPM.

Na'urar wurin kulawa tana da ƙaramin ƙaramin haske mai haske mai ƙarfi. Software na binciken hoto na musamman da aka haɓaka yana gano ƙwayoyin ƙwayoyin cuta guda ɗaya, waɗanda ke ba da damar ƙidaya ƙwayoyin da aka kama don sadar da sakamako mai ƙima. Ana motsa hasken wuta ta amfani da LEDs, waɗanda aka liƙa a ƙarƙashin katun da ke ɗauke da tashoshi na ruwa.

Yawancin lokaci, ana gano ƙwayoyin DNA da aka yi niyya ta hanyar takamaiman alamun haske. Sabuwar hanyar tana amfani da eriya tare da beads masu girman nanometer, waɗanda ke haɓaka siginar gani na waɗannan alamomin kuma suna kawar da dogaro akan haɓaka sinadarai ta hanyar PCR.

"Antenna na gani sun ƙunshi barbashi na ƙarfe na nanometer waɗanda ke ba da haske a cikin ƙaramin yanki kuma suna taimakawa wajen fitar da hasken - kamar yadda eriya na macroscopic ke yi da igiyoyin rediyo," in ji Hauer. Barbashi na ƙarfe suna da alaƙa da sinadarai zuwa saman guntu.

Tsarin kwayoyin halittar DNA, wanda masu binciken suka rarraba a matsayin DNA origami, yana rike da duka nanoparticles na gwal a wurin. Tsakanin nanoparticles, tsarin yana samar da wurin ɗaure don kwayoyin manufa daban-daban da alamar haske. Ƙirar da aka ƙirƙira tana ba da tushe don fasahar tantance sabon labari.